iPSC generation using latest traceless methods
|Reprogramming into iPS cells and correction (mRNA and CRISPR)||Cost per sample/patient||
Deliverables / notes
|6+ fibroblast samples to convert into iPSCs with basic characterisation option (karyotype/IF shown above), per sample||$3,490*||3 clones/patient, typically >50 generated and 6 initially grown;
With basic characterisation service (option 1) – after RNA reprogramming mTeSR bulk cultures of 3 clones deliverable, characterisation by karyotyping/pluripotency marker analysis by immunofluorescence
|6+ mutation correction in fibroblast samples (coupled with the above reprogramming into iPSCs), per sample/mutation||$3,290**||Includes custom CRISPR tool-set creation, generation of a larger number of clones to screen and identification of homo/heterozygously-corrected and otherwise edited clones, recommended coupling with further functionalisation of the edited clones by introduction of an off-the-shelf o=r custom targeted reporter construct, ask us for more details|
|An in-depth characterisation of iPSC clonal lines package||$1500/clone||Detailed KaryoStat™ karyotyping (array-based CNV detailed genomic map) and PluriTest (transcriptomic analysis of iPS cells and whole transcriptome-based assessment of pluripotency)|
|* for smaller and larger (24+) cohorts please contact us for pricing
** the cost of gene editing might include additional targeting construct synthesis charges, etc.
We offer iPSC generation using the latest RNA-based methods, offering an efficient, footprint-free production of iPS cells from, e.g., normal skin fibroblasts. This safe and benign method could be combined with gene correction or other types of genetic manipulation. Due to our BPA/NCRIS funding, we are able to offer a very competitive pricing on various characterization packages which could be tailored to fit your scientific and publication-compliance needs.
< A passage 1 hiPSC colony, RNA-reprogrammed from a dermal fibroblast, immediately after its transfer onto the Matrigel matrix in the mTeSR1 medium.