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MG RNA-reprog colony BF square

iPSC generation using an RNA-based reprogramming method

$ 1.00

We offer iPSC generation using the latest RNA-based methods, offering an efficient, footprint-free production of iPS cells from, e.g.,  normal skin fibroblasts. This safe and benign method could be combined with gene correction or other types of genetic manipulation. Due to our BPA/NCRIS funding, we are able to offer a very competitive pricing on various characterization packages which could be tailored to fit your scientific and publication-compliance needs.

A passage 1 hiPSC colony, RNA-reprogrammed from a dermal fibroblast, immediately after its transfer onto the Matrigel matrix in the mTeSR1 medium.

V  An example of the high-resolution karyotyping by array-based CNV determination of one of the iPS clones (male) generated using the mRNA-based reprogramming method.

Karyostat RepRNA example male (Flo#1)

Reprogramming into iPS cells and correction (mRNA and CRISPR) Cost per sample/patient Deliverables / notes
6+ fibroblast samples to convert into iPSCs with characterisation Option 1, per sample $2,990 3 clones/patient, typically >50 generated and 6 initially grown;
With basic characterization service (option 1) –  after RNA reprogramming mTeSR bulk cultures of 3 clones deliverable, characterisation by karyotyping/pluripotency marker analysis by immunofluorescence
6+ mutation correction in fibroblast samples (coupled with the above reprogramming into iPSCs), per sample $2,490+ Includes custom CRISPR toolset creation, entails generation of a larger number of clones to screen and identify homo/heterozygously-corrected clones
Option 2: an  in-depth characterisation of iPSC clonal lines package $900/clone Detailed hi-res KaryoStat™ karyotyping (array-based CNV detailed genomic map) + PluriTest (transcriptomic analysis of iPS cells and whole trsnscriptome-based assessment of pluripotency)
Estimated total service cost/patient, Options 1/2 (with 2 clones) $5,440+/$7,240+   +: the cost of gene editing might include additional construct synthesis charges, etc.

ADDITIONAL DETAILS