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Teratoma Assay

$ 1.00
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    • StemCore must be given at least 3 weeks of notice before injections.
    • Cells must be mycoplasma tested following at least 1 week of culture in antibiotic-free medium.
    • 1xT25 (or 2 x 6cm dishes) of the cell line at ~50% confluence is required per injection.
    • Teratoma formation can take anywhere from 8‐14 weeks. If no formation of a teratoma is seen at 14 weeks, StemCore would
    • recommend repeating the experiment. Customers will be invoiced for any experimental costs that have been accumulated, even in the event of no teratoma formation.

    • As standard practice, StemCore normally perform the assay in duplicate (2 mice per cell-line) but if a triplicate experiment is require please state this in your request.
    • StemCore typically injects mice aged between 4‐8 weeks. If a customer orders mice but the cells are not ready for the specified date, the customer may choose to use the mice at a later date, therefore older mice, or new mice can be ordered. Any unused mice, however, will be charged to the customer.

    Teratoma Assay includes:

    • Intramuscular injection of cells into NOD/SCID mice (2‐3 mice per cell line, at the discretion of the customer)
    • Observation of mass formation for 8-12 weeks
    • Fixing of any masses dissected from one or more of the mice (10% Formalin)
    • Embedding, sectioning and processing into H&E-stained slides
    • Evaluation of observed cell types and their representation of all three embryonic-germ cell lineages
    • Results are provided in the form of a report including images of representative cell types
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Product Description

Teratoma Assay

StemCore’s teratoma formation assay involves the intramuscular injection of stem cells into the hind leg of an immunodeficient mouse. This assay is utilised to demonstrate whether a cell line has pluripotent differentiation potential and, therefore, the ability to form all 3 embryonic germ layers in vivo.  Please read our Terms and Conditions to gain further information about the procedure and requirements of this assay.