We have substantial experience with the use of numerous modifications of Cas9 protein which have been recruited to perform the tasks of either activation and repression of the desired gene targets, as well as deposition or interpretation of the epigenetic environment of the region-of-choice, mediated by either DNA or histone modification.
Inhibition of DYRK1A disrupts neural lineage specification in human pluripotent stem cells. 2017. eLife
Bellmaine SF, Ovchinnikov DA, Manallack DT, Cuddy CE, Elefanty AG, Stanley EG, Wolvetang EJ, Williams SJ, Pera MF.
Variability of Gene Expression Identifies Transcriptional Regulators of Early Human Embryonic Development. 2015. PLoS Genetics
Hasegawa Y, Taylor D, Ovchinnikov DA, Wolvetang EJ, de Torrenté L, Mar JC.
The Impact of APP on Alzheimer-like Pathogenesis and Gene Expression in Down Syndrome iPSC-Derived Neurons 2018. Stem Cell Reports
Ovchinnikov DA, Korn, O, Virshup, I., Wells, CA, Wolvetang EJ